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Ramesh Subramanian,Assistant Professor PhD Professor (LSU-SVM)
Post Doctoral LSUHSC/LSUSVM Baton Rouge 10/05 Cancer and Virology
Post Doctoral University of Illinois, College of Med at Peoria 10/03 Cancer Therapeutics
PhD Osmania University, Hyderabad, India 07/97 Genetics
MSc Osmania University, Hyderabad, India 06/96 Genetics
B.Sc. Osmania University, Hyderabad, India 05/94 Botany Zoology Chemistry
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Personal Statement

  • I have had considerable experience in cancer biology and molecular biology. I have recently completed postdoctoral training in molecular virology working on Kaposi's sarcoma herpesvirus (KSHV) and human herpes simplex viruses. Previously I have extensively worked with siRNA mediated inhibition of breast cancer proliferation. As Assistant director of the Division of Biotechnology and Molecular Medicine, I have devoted considerable time on next generation sequencing and quantitative PCR approaches to elucidate host-pathogen interactions pursued by CEIDR researchers. I have served as program coordinator for LSU-Tulane COBRE In addition, I am directly involved in the analysis of NGS data for CEIDR researchers in the GeneLab Core Laboratory (GeneLab) and I also assist researchers in the execution of digital PCR experiments. I have extensive experience working on HSV infections studies of Ganglionic neurons and Proximity ligation assays. I am well qualified to assist in the molecular biological studies as identified in the proposal.

  • 1.Kim IJ, Saied AA, Chouljenko VN, Subramanian R, Kousoulas KG. Functional hierarchy of herpes simplex virus type-1 membrane proteins in corneal infection and virus transmission to ganglionic neurons. Curr Eye Res. 2014 Dec;39(12):1169-77.

  • 2.Charles AS, Chouljenko VN, Jambunathan N, Subramanian R, Mottram P, Kousoulas KG. Phenylalanine residues at the carboxyl terminus of the herpes simplex virus 1 UL20 membrane protein regulate cytoplasmic virion envelopment and infectious virus production. J Virol. 2014 Jul;88(13):7618-27.

  • 3.Saied AA, Chouljenko VN, Subramanian R, Kousoulas KG. A replication competent HSV-1(McKrae) with a mutation in the amino-terminus of glycoprotein K (gK) is unable to infect mouse trigeminal ganglia after cornea infection. Curr Eye Res. 2014 Jun;39(6):596-603.

  • 4.Jambunathan N, Chouljenko D, Desai P, Charles AS, Subramanian R, Chouljenko VN, Kousoulas KG. Herpes simplex virus 1 protein UL37 interacts with viral glycoprotein gK and membrane protein UL20 and functions in cytoplasmic virion envelopment. J Virol. 2014 Jun;88(11):5927-35.

  • 5.Jambunathan N, Chowdhury S, Subramanian R, Chouljenko VN, Walker JD, Kousoulas KG. Site-specific proteolytic cleavage of the amino terminus of herpes simplex virus glycoprotein K on virion particles inhibits virus entry. J Virol. 2011 Dec;85(24):12910-8.

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Positions and Honors
Project Assistant, Indian Institute of Science, Bangalore, India. 1996-1997
Post Doctoral Research Associate, Univ. of Illinois, College of Medicine, Peoria 2003-2005
Post Doctoral Research Associate, LSUHSC New Orleans 2005- 2006
Staff Scientist, SUBR/LSU School of Vet. Med., Baton Rouge 2006- 2012
Assistant Director Div of BIOMMED/Coordinator CEIDR 2012-present
Assistant Professor Research, Division of BIOMMED (LSUSVM) 2014-present
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Other Experience and Professional Memberships

  • Member AACR 2003-2014

  • Member of the RNA Society (www.rnasociety.org).

  • Member of the Science Advisory Board (http://scienceboard.net)

  • I am an editorial board as technical editor for the following online journals, Biotechnology, International Journal of Virology, Trends in Molecular Sciences

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Awards received
Junior Research Fellow Rockefeller Foundation, New York, USA
Senior Research Fellow Council for Scientific and Industrial Research, New Delhi, Govt. of India
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Contributions to Science

  • 1.My early training was in the field of applied molecular genetics in rice. I have developed a novel technique for agrobacterium based transformation of stem-borer resistance and plant hopper resistance in parental lines of the Hybrid rice program as well as high yielding popular rice varieties. The resultant transformed rice lines showed high degree of tolerance to yellow stemborers as well as brown plant hopper in field trials

  • a. Ramesh S, Nagadhara D, Reddy VD and Rao KV (2004). Production of transgenic indica rice resistant to yellow stem borer and sap-sucking insects, using super binary vectors of Agrobacterium tumefaciens. Plant Science. 166:1077-1085.

  • b. Ramesh S, Nagadhara D, Padmakumari APK, Pasalu IC, Sarma NP, Reddy VD and Rao KV (2004). Development of stem borer resistant transgenic parental lines involved in the production of hybrid rice. J Biotechnol. 111(2):131-41

  • c. Nagadhara D, Ramesh S, Pasalu IC, Rao KY, Krishnaiah NV, Sarma NP, Reddy VD and Rao KV (2004). Transgenic rice plants expressing the snowdrop lectin gene ( gna) exhibit high-level resistance to the whitebacked planthopper ( Sogatella furcifera). (Theoretical and Applied Genetics). 109(7):1399-405

  • d.Nagadhara D, Ramesh S, Pasalu IC, Rao KY, Krishnaiah NV, Sarma NP, Bown DP, Gatehouse JA, Reddy VD and Rao KV (2003). Transgenic indica rice plants resistant to sap sucking insects. Plant Biotechnology Journal 1 (3): 231-240.

  • 2. During my postdoctoral studies at University of Illinois College of Medicine at Peoria, I worked on the development of novel siRNA based therapeutics for breast cancer. Urokine Plasminogen activator and its receptor play an important role in proliferation and metastasis of breast cancer cells. UPA-r also plays an important role in regulating intracellular signaling. I used siRNAs targetting UPA and its receptor to inhibit the proliferation of breast cancer cells.

  • a. Subramanian, R., C. S. Gondi, S. S. Lakka, A. Jutla, J. S. Rao . 2006. siRNA-mediated simultaneous downregulation of uPA and its receptor inhibits angiogenesis and invasiveness triggering apoptosis in breast cancer cells Int J Oncol. Apr;28(4):831-9.

  • 3. Kaposis's sarcoma and B Cell lymphomas are caused by Human Herpes Virus * or Kaposi's Sarcoma Associated Herpesvirus. I generated plasmid vectors expressing shRNAs targeting the envelope glycoprotein gB and K8.1 of KSHV to study the effects on tumor related angiogenesis and the associated signaling. Interestingly down regulation of gB and K8.1 not only led to inhibition of angiogenic proteins in BCBL-1 and endothelial cells but also led to downregulation of KSHV lytic gene expression.

  • a. Subramanian, R., I. Sehgal , O. D'Auvergne, and K. G. Kousoulas KG. 2010. Kaposi's Sarcoma-associated herpesvirus (KSHV) viral glycoproteins B (gB) and K8.1 regulate virion egress and VEGF and vIL-6 synthesis in BCBL-1 cells. J Virol. Feb;84(4):1704-14.

  • b. Subramanian, R., O. D'Auvergne O, H. Kong H, and K. G. Kousoulas. 2008. The cytoplasmic terminus of Kaposi's sarcoma-associated herpesvirus glycoprotein B is not essential for virion egress and infectivity. J Virol. Jul;82(14):7144-54.

  • 4. HSV-1 Glycoprotein K plays an important role in regulating entry of HSV1 into neurons via the ganglia. We have demonstrated that gK is part of the HSV1 virion and mutant HSV-1 lacking 68 aa from the amino terminus of gK are trapped on the surface and unable to enter ganglionic axons.

  • a. Kim IJ, Saied A A, Chouljenko VN, Subramanian R, Kousoulas KG (2013). Functional Hierarchy of Herpes Simplex Virus Type-1 (HSV-1) Membrane Proteins in Corneal Infection and Virus Transmission to Ganglionic Neurons. (Curr Eye Res. Manuscript under review.)

  • b. Saied A A, Chouljenko VN, Subramanian R, Kousoulas KG (2013) In press. A Replication Competent HSV-1(McKrae) with a Mutation in the Amino-terminus of Glycoprotein K (gK) Is Unable to Infect Mouse Trigeminal Ganglia after Cornea Infection. (Curr Eye Res. 2013)

  • c. David A T; Saied A A; Charles A S; Subramanian R; Chouljenko V N; Kousoulas K G 2012. A Mutant Herpes Simplex Virus Type-1 (HSV-1) McKrae Lacking the Glycoprotein K (gK) Gene is Unable to Infect Neuronal Axons and Egress from Neuronal Cell Bodies. MBio. 2012 Jul 24;3(4):e00144-12. doi: 10.1128/mBio.00144-12.

  • d. Jambunathan, N., S. Chowdhury, R. Subramanian, V. N. Chouljenko, J. D. Walker, and K. G. Kousoulas. 2011. Site-specific cleavage of the amino-terminus of herpes simplex virus type-1(HSV-1) glycoprotein K (gK) on virion particles inhibits virus entry. J. Virol. doi:10.1128/JVI.06268-11.

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Complete List of Published Work in MyBibliography:
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Ongoing Research Support

  • Role: Co-Investigator (KG Kousoulas PI)

  • Governor’s Biotechnology Initiative Program Grant (Kousoulas, Konstantin G.) 10/24/12-06/30/14. Louisiana Board of Regents. New cancer treatment modalities. This overall program project focuses on the use of adenoviruses and herpesviruses for the expression of specific lymphokines, transcriptional factors and other genes for the treatment of tumors in a mouse model.

  • NIH:NIGMS P20 RR020159-10 (NCE) Kousoulas (PI) 05/01/2009 – 04/30/2014 Role:

  • Program Coordinator: This application funds a Center of Biomedical Research Excellence (COBRE) on Experimental Infectious Disease Research (CEIDR) at the Louisiana State University, School of Veterinary Medicine (LSUSVM) in collaboration with the Tulane National Primate Research Center (TNPRC). The application contains five projects led by assistant professors.

Support of the NIH and NIGMS P20GM103458-10 and 8P20GM103424 is gratefully acknowledged.
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